论文部分内容阅读
目的 研究葡萄糖激酶 (GCK)基因启动子 - 30G/A变异在中国南方地区汉族人群中的分布并比较其在正常糖耐量 (NGT)者及糖耐量低减 (IGT)者中的差别及其与糖代谢相关指标之间的关系。方法 研究对象为 4 4 4例无糖尿病及亲缘关系的中国南方地区汉族人 ,其中 2 2 2例为NGT ,2 2 2例为IGT。通过PCR RFLP检测GCK启动子 - 30位置的G→A变异所导致BsiHKAⅠ酶切位点的缺失。结果 在IGT及NGT的人群中含有A等位基因的频率分别为 18.2 %及 19.8%。与GG基因型的人群相比 ,带有A等位基因的人群空腹血糖显著增高〔(5 .35± 0 .5 9vs 5 .19± 0 .5 3)mmol/L ,P =0 .0 0 6〕 ,在IGT的人群中 ,带有A等位基因的人群空腹血糖仍显著增高〔(5 .5 4± 0 .5 8vs 5 .35± 0 .5 2 )mmol/L ,P =0 .0 2〕 ,而在NGT的人群中 ,不同GCK基因型者的空腹血糖差异无显著性。结论GCK基因启动子 - 30G/A变异与空腹高血糖水平相关 ,可能对中国人的IGT有一定的影响。
Objective To investigate the distribution of the 30G / A variant of glucokinase (GCK) gene promoter in Chinese Han population of southern China and to compare the difference between normal glucose tolerance (NGT) and impaired glucose tolerance (IGT) The relationship between glucose metabolism related indicators. METHODS: Forty-four Han Chinese with no diabetes and their relatives were enrolled in this study. Among them, 222 were NGT and 222 were IGT. Deletion of the BsiHKA I restriction site was detected by PCR RFLP for the G → A variation of GCK promoter -30. Results The frequencies of A allele in IGT and NGT populations were 18.2% and 19.8% respectively. Compared with people with GG genotype, fasting blood glucose was significantly higher in people with the A allele (5 35 ± 0.59 vs 5 19 ± 0 53 mmol / L, P 0 0 0 0 6]. In the IGT population, fasting blood glucose was still significantly elevated in people with the A allele (5.54 ± 0.58 vs 5.35 ± 0.52 mmol / L, P = 0). 0 2〕, but in NGT population, different GCK genotypes had no significant difference in fasting blood glucose. Conclusion The GCG gene promoter - 30G / A mutation is associated with fasting hyperglycemia and may have some effect on Chinese IGT.