论文部分内容阅读
目的探讨基因芯片法快速检测结核分枝杆菌(MTB)评估利福平和异烟肼耐药性的价值。方法收集283例结核患者的痰液标本,采用罗氏固体培养绝对浓度法对MTB进行传统药敏试验。应用晶芯MTB耐药检测试剂盒对MTB利福平耐药相关基因rpoB的rpoB-RRDR 511、513、516、526、531和533位点以及异烟肼耐药相关基因katG315、inhA-15位点进行检测,比较两种检测方法的结果。结果与罗氏法相比,基因芯片法对菌株利福平药敏检测的灵敏度为83.33%,特异度为98.42%,对菌株异烟肼药敏检测的灵敏度为89.19%,特异度为97.15%。结论采用基因芯片法对rpoB基因和katG基因相关位点的检测可为临床评估结核病患者利福平和异烟肼耐药提供一种快速、准确和批量性的检测手段。
Objective To investigate the value of rapid detection of Mycobacterium tuberculosis (MTB) in evaluating the drug resistance of rifampin and isoniazid by gene chip. Methods Totally 283 sputum samples of tuberculosis patients were collected. The traditional drug susceptibility test of MTB was carried out by Roche solid culture absolute concentration method. 511, 513, 516, 526, 531 and 533 of the rpoB-RRDR gene of rpoB, and isoniazid resistance-related genes katG315, inhA-15 Site for testing, comparing the results of two test methods. Results Compared with the Roche method, the sensitivity and the specificity of the gene chip method for the determination of rifampicin were 83.33% and 98.42%, respectively. The sensitivity and specificity of isoxazole were 89.19% and 97.15%, respectively. Conclusion The detection of rpoB gene and katG gene related sites using gene chip method can provide a rapid, accurate and batch detection method for clinical evaluation of rifampicin and isoniazid resistance in patients with tuberculosis.