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采用同位素掺入法,以同位素标记物掺入速率为指标,观察了鳖血清对艾氏腹水癌细胞和正常小鼠骨髓细胞的DNA、RNA和蛋白质生物合成的影响,结果表明鳖血清可强烈地抑制~3H-TdR、~3H-UR和~3H-Leu掺入癌细胞,其抑制率分别为96.8％、94.8％和91.9％,而对~3H-TdR和~3H-UR掺入骨髓细胞的抑制率为39.4％和37.3％。将鳖血清在50℃加热十分钟,其抑制同位素标记物掺入靶细胞的活性几乎全部丢失。但鳖血清在生理盐水中透析不影响它的抑制活性。这些结果提示,鳖血清中存在某种不耐热的组份,可强烈抑制癌细胞生长,同时对正常骨髓细胞也有一定抑制作用。
The isotope incorporation method was used to measure the DNA, RNA, and protein biosynthesis of Ehrlich ascites carcinoma cells and normal mouse bone marrow cells using the isotope label incorporation rate as an indicator. The results showed that the cerium serum can strongly Inhibition of ~3H-TdR, ~3H-UR, and ~3H-Leu incorporation into cancer cells was 96.8%, 94.8%, and 91.9%, respectively, whereas the incorporation of ~3H-TdR and ~3H-UR into bone marrow cells The inhibition rates were 39.4% and 37.3%. Heating quail serum at 50°C for ten minutes resulted in almost complete loss of activity in inhibiting incorporation of isotopic markers into target cells. However, dialysis of sputum serum in physiological saline does not affect its inhibitory activity. These results suggest that the presence of certain thermolabile components in the serum of quail can strongly inhibit the growth of cancer cells and also have a certain inhibitory effect on normal bone marrow cells.