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目的探讨乙酰肝素酶与大肠癌浸润、转移和血管生成之间的关系。方法应用原位杂交方法检测乙酰肝素酶mRNA在95例大肠癌组织中的定位及表达。并用免疫组化方法对全部标本进行CD105染色,记数肿瘤微血管密度(microvessel density,MVD),分析乙酰肝素酶mRNA表达与大肠癌浸润、转移和MVD之间的关系。结果95例大肠癌组织中,乙酰肝素酶mRNA阳性表达49例(51.57%),MVD平均值为(72.1±20.6);阴性表达46例(48.42%),MVD平均值为(41.3±12.4),差异有统计学意义(P<0.01)。乙酰肝素酶mRNA表达与大肠癌组织浸润深度、淋巴结转移及MVD有关(P<0.05)。结论乙酰肝素酶可促进大肠癌的浸润、转移和血管生成,可作为反映大肠癌生物学行为的客观指标。
Objective To investigate the relationship between heparanase and invasion, metastasis and angiogenesis in colorectal cancer. Methods The in situ hybridization was used to detect the localization and expression of heparanase mRNA in 95 colorectal cancer tissues. All specimens were stained with CD105 by immunohistochemistry, and the tumor microvessel density (MVD) was recorded. The relationship between heparanase mRNA expression and the invasion, metastasis and MVD in colorectal cancer was analyzed. Results The positive expression of heparanase mRNA was found in 49 cases (51.57%) of 95 cases of colorectal cancer, the average MVD was (72.1 ± 20.6), 46 cases (48.42%) were negative, the average MVD was (41.3 ± 12.4) , The difference was statistically significant (P <0.01). The heparanase mRNA expression was correlated with the depth of invasion, lymph node metastasis and MVD in colorectal cancer (P <0.05). Conclusion Heparanase can promote the invasion, metastasis and angiogenesis of colorectal cancer, which can be used as an objective index to reflect the biological behavior of colorectal cancer.