Objective: To determine the level of dengue prevention practices among wet market traders in a Malaysian district and their associated socio-ecological factors including individual, relationships, community and societal factors. Methods: A cross-sectional study involving 246 wet market traders was conducted in a district in Perak state in northwest Malaysia between September 2018 to June 2019. Participants were selected through stratified sampling from four wet markets in Hilir Perak district. Data on dengue prevention practices and associated socio-ecological characteristics were collected using a validated interview-based questionnaire. The data were analyzed using SPSS version 25. Multiple logistic regression was performed to identify socio-ecological determinants of dengue prevention practices among wet market traders in Hilir Perak District. Results: From the total number of respondents, 78％ had high dengue prevention practices. Higher dengue prevention practices were associated with owners of wet market shoplots compared to employed workers (adjusted OR 4.18, 95％ CI 1.78, 9.85), high perceived susceptibility (adjusted OR 6.93, 95％ CI 3.02, 15.92), high familial support (adjusted OR 3.65, 95％ CI 1.25, 10.64), and high perceived dengue prevention and control laws and regulations (adjusted OR 3.24, 95％ CI 1.44, 7.32). Conclusions: Dengue prevention practices were associated not only with individual determinants but also with other determinants from relationships to societal level which must be considered in planning or evaluating current dengue control programs.