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目的:建立去甲氧基荚果蕨素的含量测定方法,并测定不同地区商品贯众中去甲氧基荚果蕨素的含量。方法:HPLC法,YWG-C18色谱柱(4.6 mm×250 mm,10μm),流动相甲醇-水(78∶22)等度洗脱,流速1.0 mL·min-1,检测波长为298nm,柱温25℃。结果:去甲氧基荚果蕨素进样量在0.188 0～1.316μg时线性关系良好,r=0.999 7,平均回收率为99.38%,RSD 2.40%。对不同地区商品贯众中去甲氧基荚果蕨素的含量测定结果表明,河北石家庄的绵马贯众中去甲氧基荚果蕨素的含量最高,为0.35%,北京西城的绵马贯众中去甲氧基荚果蕨素的含量最低,为0.17%。结论:该方法操作简单,快速,准确,重复性好,可用于商品贯众及其相关药材中去甲氧基荚果蕨素的含量分析。
Objective: To establish a method for determination of demethoxy pod fern and to determine the content of demethoxy pod fern in commercial products of different regions. Methods: HPLC method was used on a YWG-C18 column (4.6 mm × 250 mm, 10 μm). The mobile phase was eluted with isocratic elution of methanol-water (78:22) at a flow rate of 1.0 mL · min- 25 ° C. Results: The linearity of demethoxy pod fern injection was good at 0.188 0-1.316 μg, r = 0.999 7, the average recovery was 99.38% and RSD was 2.40%. Determination of the content of desmethoxy pod fern in the products of different regions showed that the content of desmethoxy pod fern in Mianma Guanzhong of Shijiazhuang was the highest at 0.35% The content of desmethoxy pod fern was the lowest, 0.17%. Conclusion: The method is simple, rapid, accurate and reproducible. It can be used for the determination of desmetoxypyran fern in commercial products and its related medicinal materials.