以斑马鱼AB品系囊胚晚期胚胎细胞为细胞核供体,以斑马鱼长鳍品系未受精的去核卵为受体,进行不同品系间斑马鱼的细胞核移植.采用显微注射法,操作胚胎1119枚,获得克隆鱼14尾.RAPD分析显示,克隆鱼与细胞核供体鱼的DNA扩增带纹一致而与受体鱼不同,表明克隆鱼的遗传物质来源于供体细胞核.模式动物斑马鱼的细胞核移植技术的建立,有望在研究动物发育过程的基因功能、细胞核的发育潜能及核质关系等重要问题上发挥作用. The zebrafish AB line blastocysts were used as the nucleus donor, and the zebra fish unfertilized enucleated eggs were used as the acceptors for the nuclear transplants of zebrafish from different strains.Using the microinjection method, the embryos 1119 And obtained cloned fish 14. RAPD analysis showed that the clone fish and nuclear donor fish DNA stripe pattern consistent with the recipient fish, indicating that the genetic material of the cloned fish from the donor cell nucleus. The establishment of nuclear transfer technology is expected to play an important role in the study of gene function, the developmental potential of nucleus and the relationship between nuclear and nuclear in animal development.