有报道认为,光能抑制黄连细胞的生长和黄连素的产生,而充气则有促进作用。植物细胞培养表明,变异性和特异性是由培养细胞自身引起的遗传性变异。为此,研究了产生大量黄连素的黄连细胞代谢的调节和细胞系的选择。取日本黄连(Coptis japonica Makiao Var.dis-secta Nakai)的小根碎片,于液状L-S 培养基中,于26±1℃暗处培养,选择外观均匀、无根的转移到斜面 It has been reported that light can inhibit the growth of Coptis chinensis and the production of berberine, while aeration can promote it. Plant cell cultures show that the variability and specificity are genetic variations caused by the cultured cells themselves. For this reason, the regulation of cell metabolism and the selection of cell lines of Coptis chinensis yielding a large amount of berberine were studied. The small root fragments of Coptis japonica Makiao Var.dis-secta Nakai were cultured in liquid L-S medium and cultured in the dark at 26±1°C. Uniform and non-rooted transfer to bevel was selected.