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目的:比较Piroxicam(环氧化酶-1选择抑制剂)和NS-398(环氧化酶-2选择性抑制剂)对角膜新生血管的抑制作用并比较两者对角膜上皮细胞的毒性作用。方法:将含20μg脂多糖的缓释小丸植入兔角膜基质以诱导角膜新生血管。使用不同浓度的Piroxi-cam和NS-398滴眼剂滴眼,植入10d后分析角膜新生血管的面积。采用MTT法分析Piroxicam和NS-398对角膜上皮细胞的毒性作用。结果:0.01μmol/LNS-398和0.01μmol/LPiroxi-cam组兔角膜新生血管生长浓密,其它组因抑制剂的不同及浓度的不同角膜新生血管面积有不同程度的减低。在0.5,5及50μmol/L时,NS-398较Piroxicam对角膜上皮细胞的毒性低。结论:环氧化酶-1特异性抑制剂和环氧化酶-2特异性抑制剂对角膜新生血管形成均有抑制作用。相同浓度时,NS-398的抑制效果强于Piroxicam。在高浓度时NS-398较Piroxicam对角膜上皮细胞的毒性低。
OBJECTIVE: To compare the inhibitory effects of Piroxicam (selective inhibitor of cyclooxygenase-1) and NS-398 (selective inhibitor of cyclooxygenase-2) on corneal neovascularization and to compare their toxic effects on corneal epithelial cells. Methods: The sustained-release pellets containing 20 μg lipopolysaccharide were implanted into corneal stroma of rabbits to induce corneal neovascularization. Different concentrations of Piroxi-cam and NS-398 eye drops were used to assess corneal neovascularization area after 10 days of implantation. The toxic effects of Piroxicam and NS-398 on corneal epithelial cells were analyzed by MTT assay. Results: The corneal neovascularization was thick in 0.01μmol / L NS-398 and 0.01μmol / LPiroxi-cam groups, while the other groups had different degrees of corneal neovascularization due to different inhibitors and concentrations. At 0.5, 5 and 50 μmol / L, NS-398 was less toxic to corneal epithelial cells than Piroxicam. Conclusion: Cyclooxygenase-1 specific inhibitors and cyclooxygenase-2 specific inhibitors have inhibitory effects on corneal neovascularization. At the same concentration, the inhibitory effect of NS-398 was stronger than that of Piroxicam. NS-398 is less toxic to corneal epithelial cells than Piroxicam at high concentrations.