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为了解慢性肝病患者间质胶原酶基因表达的变化 ,选择慢性肝炎 12例、肝硬化 13例及正常对照 8例 ;用逆转录定量PCR方法 ,测定慢性肝病患者间质胶原酶的基因表达水平。结果表明正常人有间质胶原酶(MMP1)的基因表达 ;慢性肝炎、肝硬化患者MMP1基因表达较正常对照明显增强 ;慢性肝炎患者MMP1基因表达水平明显高于肝硬化患者。上述结果提示慢性肝病患者间质胶原酶基因表达的变化可能是影响其酶活性变化的重要因素
In order to understand the change of gene expression of interstitial collagenase in patients with chronic liver disease, 12 cases of chronic hepatitis, 13 cases of cirrhosis and 8 cases of normal control were selected. The gene expression of interstitial collagenase in patients with chronic liver disease was determined by reverse transcription polymerase chain reaction (RT-PCR). The results showed that the normal human interstitial collagenase (MMP1) gene expression; chronic hepatitis, liver cirrhosis patients with MMP1 gene expression was significantly enhanced compared with the normal control; MMP1 gene expression in patients with chronic hepatitis was significantly higher than in patients with cirrhosis. These results suggest that changes in the expression of interstitial collagenase gene in patients with chronic liver disease may be an important factor affecting the changes of its enzyme activity