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目的分析广东汉族人群RhD阴性个体中,RhDel表型个体RHEC表型的分布情况和两者的相关性,以及PCR-SSP技术在RhDel基因检测中的应用价值。方法对400份广东汉族人群中血型血清学检测为RhD阴性的标本,采用血型血清学方法对RhDel表型和RHEC表型进行检测,并采用PCR-SSP方法进行RHD1227G>A基因检测。结果在400份RhD阴性标本中,89例(22.25%)检测到RhDel表型,主要分布在CCee、CeEe和Ccee表型的个体,在CCEe、ccEE、ccEe和ccee表型中未发现。在RhDel表型的标本中,同时检测到RHD1227G>A基因。结论 RhDel表型与RHEC表型具有一定的相关性;应用PCR-SSP技术能够对RhDel基因进行检测;与传统的间接抗人球蛋白实验和吸收放散实验相比,PCR-SSP技术能够快速、便捷筛查RhDel献血者和受血者,避免抗-D抗体引发的同种免疫反应和RhD阴性血液的浪费。
Objective To analyze the distribution of RHEC phenotypes in RhD negative individuals and their correlations in RhD negative individuals of Guangdong Han population and the application value of PCR-SSP in RhDel gene detection. Methods RhD negative phenotype was detected in 400 Han population in Guangdong. RhDel phenotype and RHEC phenotype were detected by serological method, and RHD1227G> A gene was detected by PCR-SSP. RESULTS: Of the 400 RhD-negative specimens, 89 (22.25%) detected RhDel phenotypes, which were mainly found in individuals with CCee, CeEe and Ccee phenotypes and were not found in the CCEe, ccEE, ccEe and ccee phenotypes. RHD1227G> A gene was also detected in RhDel phenotype samples. Conclusion RhDel phenotype has some correlation with RHEC phenotype; PCR-SSP technology can detect RhDel gene; PCR-SSP technology can be fast and convenient compared with the traditional indirect anti-human globulin experiment and absorption and desorption experiments Screen RhDel blood donors and recipients to avoid alloimmunization induced by anti-D antibodies and wasting of RhD-negative blood.