激光捕获显微切割是一种先进的分离特定同质细胞群进行进一步研究的技术,尤其是在需要研究的细胞占样本细胞数很少时,以及需研究的细胞呈散在分布时,激光捕获显微切割的重要性尤为明显。与免疫组织化学、原位杂交技术、高通量基因分析、蛋白分析技术结合,显微切割技术显示出良好的发展前景。本文简述激光捕获显微切割的基本原理、应用以及可能的发展趋势。 Laser capture microdissection is an advanced technique for further research on the isolation of specific homogeneous cell populations, especially when the number of cells in the sample to be studied is small, and when the cells to be studied are scattered, The importance of microdissection is particularly evident. In combination with immunohistochemistry, in situ hybridization, high-throughput gene analysis, and protein analysis techniques, microdissection shows a promising future. This article describes the basic principles, applications and possible trends of laser capture microdissection.