用HPLC法测定三磷酸腺苷二钠滴眼液中乙酸氯己定的含量。以HypersilODS2 为分析柱 ,V( 0 .0 1mol/L磷酸二氢钾 )∶V(甲醇 ) =32∶6 8(H3 PO4 调pH至 4.30 )为流动相 ,检测波长为 2 5 4nm ,以安定为内标 ,在 2 .5～ 80 .0 μg/mL浓度范围内 ,乙酸氯己定与内标峰面积之比和浓度呈良好的线性关系 (r =0 .9998) ,平均回收率为 99.8% ,RSD为 0 .36 %。本法适用于该制剂的质量控制。 Determination of chlorhexidine acetate in sodium adenosine triphosphate eye drops by. HypersilODS2 as analytical column, V (0.1mol / L potassium dihydrogenphosphate): V (methanol) = 32:68 (H3PO4 pH to 4.30) as the mobile phase, the detection wavelength of 254nm, in order to stability As the internal standard, the linear relationship between the concentration of chlorhexidine acetate and the internal standard peak area (r = 0.9998) was obtained at a concentration range of 2.5 ~ 80.0 μg / mL with an average recovery of 99.8 %, RSD is 0.36%. This Law applies to the quality control of the preparation.