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目的探讨在夏氏疟原虫(Plasmodiumchabaudi chabaudi AS,P.c.chabaudi AS)感染早期,树突状细胞(Den-dritic cells,DCs)调控Th1细胞免疫应答的相关机制。方法用P.c.chabaudi AS感染易感型DBA/2和抵抗型BALB/c小鼠,计数红细胞感染率;感染前和感染后3、5、8d制备脾细胞悬液,磁珠分选、纯化DCs并体外培养;ELISA方法检测DCs培养上清中IL-12p40、IL-10和TGF-β1的水平。结果 DBA/2小鼠DCs培养上清中IL-12p40的水平在感染后3-8天明显升高,BALB/c小鼠DCs培养上清中IL-12p40的水平在感染后5-8d出现有意义的升高,但其水平均明显低于DBA/2小鼠;两种小鼠DCs培养上清中IL-10与TGF-β1的分泌水平在感染后5-8d明显升高,但DBA/2小鼠DCs培养上清中IL-10与TGF-β1的分泌水平明显低于BALB/c小鼠。结论 P.c.chabaudi AS感染早期,DBA/2和BALB/c小鼠在DCs细胞因子的分泌模式上存在显著差异。
Objective To investigate the mechanism of Th1 immune response by dendritic cells (DCs) during the early stage of infection with Plasmodium chabaudi AS (P.c. chabaudi AS). Methods Susceptible DBA / 2 and resistant BALB / c mice were infected with Pcchabaudi AS, and the infection rate of erythrocytes was counted. Spleen cell suspensions were prepared before and at 3, 5 and 8 days after infection. Cultured in vitro; ELISA method was used to detect the levels of IL-12p40, IL-10 and TGF-β1 in the supernatant of DCs. Results The levels of IL-12p40 in DCs supernatant of DBA / 2 mice increased significantly 3-8 days after infection, and the levels of IL-12p40 in DCs supernatant of BALB / c mice appeared 5-8 days after infection But their levels were significantly lower than that of DBA / 2 mice. The secretion of IL-10 and TGF-β1 in the culture supernatants of DCs from both mice increased significantly 5-8 days after infection, but DBA / The secretion of IL-10 and TGF-β1 in DCs supernatant of 2 mice was significantly lower than BALB / c mice. Conclusion In the early stage of P.c.chabaudi AS infection, DBA / 2 and BALB / c mice showed significant differences in the cytokine secretion patterns of DCs.