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目的:建立清火片中大黄素和大黄酚的含量测定HPLC方法。方法:采用Hypersil C_(18)(250 mm×4.6 mm,5μm)色谱柱,甲醇-0.1 mol·L~(-1)磷酸溶液(85:15)为流动相,流速为1.0 ml·min~(-1),检测波长为254 nm。结果:大黄素和大黄酚线性范围分别在2.0～12.0μg·ml~(-1)(r=0.999 9)和4.7～27.9μg·ml~(-1)(r=0.999 9);浓度范围内呈良好的线性关系,平均回收率分别为97.6%,98.2%。RSD为1.1%,0.8%(n=6)。结论:方法简便,快速、准确、可靠,可作为该制剂质量控制的方法。
Objective: To establish a HPLC method for the determination of emodin and chrysophanol in Qinghuo tablets. METHODS: A Hypersil C 18 (250 mm×4.6 mm, 5 μm) column was used. The mobile phase consisted of methanol-0.1 mol·L -1 phosphoric acid (85:15) and the flow rate was 1.0 ml·min~( -1), detection wavelength is 254 nm. Results: The linear ranges of emodin and chrysophanol were 2.0～12.0μg·ml -1 (r=0.999 9) and 4.7～27.9μg·ml -1 (r=0.999 9), respectively. A good linear relationship was observed, with average recoveries of 97.6% and 98.2%, respectively. RSD was 1.1%, 0.8% (n=6). Conclusion: The method is simple, rapid, accurate and reliable. It can be used as a method for quality control of the preparation.