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目的分析肝癌组织缺氧诱导因子(HIF)-1α和磷脂酰肌醇蛋白多糖(GPC)-3表达及其临床意义。方法以自身配对法分别收集经手术切除的36份肝癌及癌周组织,以免疫组织化学法分析肝癌及癌周组织HIF-1α和GPC-3的表达及分布,探讨其临床意义。结果肝癌及癌周组织HIF-1α和GPC-3表达呈棕黄色,颗粒状。前者主要定位于胞浆中,中央静脉周围明显;后者定位于胞浆和细胞膜。癌组织HIF-1α表达阳性率为80.6%,明显低于癌旁的100.0%(P<0.01),与肿瘤大小相关,其强度与分化程度负相关,与HBV感染、肿瘤数目及AFP浓度均无关;癌组织GPC-3阳性率为80.6%,明显高于癌旁的41.7%(P<0.01),与分化程度、肿瘤数目、AFP和HBsAg阳性与否之间未见明显相关。结论肝癌组织HIF-1α和GPC-3的异常表达反映肝癌的生物学行为,可作为诊断肝癌和浸润转移的有用指标。
Objective To analyze the expression of hypoxia-inducible factor-1α (HIF-1α) and phosphatidylinositol proteoglycan (GPC)-3 in hepatocellular carcinoma and its clinical significance. Methods Thirty-six hepatocellular carcinoma and pericancerous tissue were collected by self-pairing method. The expression and distribution of HIF-1α and GPC-3 in hepatocellular carcinoma and pericancerous tissue were analyzed by immunohistochemical method and its clinical significance was discussed. Results The expressions of HIF-1α and GPC-3 in hepatocellular carcinoma and hepatocellular carcinoma were brownish yellow and granular. The former is mainly located in the cytoplasm and is clearly around the central vein; the latter is located in the cytoplasm and the cell membrane. The positive rate of HIF-1α expression in cancer tissues was 80.6%, which was significantly lower than that in adjacent tumors (100%) (P<0.01). It was related to the size of tumors. The intensity of HIF-1α was negatively correlated with the degree of differentiation, and was not related to HBV infection, tumor number and AFP concentration. The positive rate of GPC-3 in cancer tissues was 80.6%, which was significantly higher than that in paratumor tissues (41.7%) (P<0.01). No significant correlation was found between the degree of differentiation, the number of tumors, and the positive or negative AFP and HBsAg. Conclusion The abnormal expression of HIF-1α and GPC-3 in hepatocellular carcinoma reflect the biological behavior of liver cancer, which can be used as a useful indicator for the diagnosis of hepatocellular carcinoma and invasion and metastasis.