论文部分内容阅读
目的 :研究黄芪当归合剂 (A&A)对梗阻性肾病大鼠肾小管间质纤维化的防治作用 ,探讨其抗纤维化作用的可能机制。方法 :采用单侧输尿管梗阻 (UUO)诱导肾间质纤维化的动物模型 ,以目前公认具有肾脏保护作用的血管紧张素转换酶抑制剂 (ACEI)作为阳性对照 ,将大鼠随机分为假手术组 (Sham)、模型组 (UUO)、黄芪当归合剂组 (A&A)、依那普利组 (ACEI) ,术后第 1 0天处死大鼠 ,收集血清测定肌酐、尿素氮水平 ,肾组织用Masson染色后进行图像分析评定各组肾小管间质损害程度 ,免疫组化半定量检测各组肾间质的α 平滑肌肌动蛋白 (α SMA)、转化生长因子 β1 (TGF β1 )、纤维连接蛋白 (FN)和层粘连蛋白 (LN)的表达。结果 :UUO组的肾小管间质损伤指数及α SMA、TGF β1 、FN和LN的表达明显高于Sham组 ,肾小管间质损伤指数与肾间质的α SMA、TGF β1 、FN和LN的表达均呈正相关关系。A&A组、ACEI组的肾小管间质损伤指数及α SMA、TGF β1 、FN和LN的表达均明显低于UUO组 ;两治疗组间比较 ,A&A对TGF β1 表达的抑制作用弱于依那普利的作用 ,而其余各指标差异无显著性。结论 :在单侧输尿管梗阻诱导的肾间质纤维化大鼠模型中 ,中药复方A&A的防治作用与ACEI相似 ,可能是通过减少肾间质肌成纤维细胞的数量、抑制TGF β1 表达
Objective: To study the preventive and therapeutic effects of Astragalus and Angelica mixture (A&A) on renal tubulointerstitial fibrosis in obstructive nephropathy rats, and to explore the possible mechanism of anti-fibrosis. METHODS: An animal model of renal interstitial fibrosis induced by unilateral ureteral obstruction (UUO) was used. The currently accepted renal protective effect of angiotensin-converting enzyme inhibitor (ACEI) was used as a positive control. Rats were randomly assigned to sham surgery. The rats (Sham), model group (UUO), Astragalus Angelica mixture group (A&A), and enalapril group (ACEI) were sacrificed on the 10th day after operation. The serum was collected and the creatinine and urea nitrogen levels were measured. After Masson staining, image analysis was performed to assess the degree of tubulointerstitial damage. Semi-quantitative immunohistochemical detection of interstitial α smooth muscle actin (α SMA), transforming growth factor β 1 (TGF β1 ), fibronectin in each group. (FN) and laminin (LN) expression. Results: The expression of α-SMA, TGFβ1, FN, and LN in the UUO group was significantly higher than that in the Sham group. The renal tubulointerstitial injury index and the interstitial α SMA, TGF β1, FN, and LN were significantly higher in the UUO group. There was a positive correlation between the expressions. The tubulointerstitial injury index and the expression of α SMA, TGFβ1, FN, and LN in the A&A group and ACEI group were significantly lower than those in the UUO group. Compared with the two groups, the inhibitory effect of A&A on TGFβ1 expression was weaker than that of enalap The role of Lee, while the remaining indicators were not significantly different. Conclusion: In the rat model of unilateral ureteral obstruction-induced renal interstitial fibrosis, the preventive and therapeutic effects of the Chinese compound compound A&A were similar to those of ACEI, probably by reducing the number of renal interstitial muscle fibroblasts and inhibiting the expression of TGFβ1.