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目的：研究细胞间粘附分子－１（ＩＣＡＭ－１）反义ＤＮＡ对内皮细胞ＩＣＡＭ－１表达的抑制作用。方法：构建ＩＣＡＭ－１反义ＤＮＡ载体，用脂质体转染内皮细胞，ＴＮＦα刺激后，流式细胞术检测转染细胞表面ＩＣＡＭ－１蛋白的表达。结果：酶切鉴定证明，１．４ｋｂ的ＩＣＡＭ－１ＤＮＡ片段反向连接到ｐｃＤＮＡ３表达载体；流式细胞术检测显示，正常细胞加ＴＮＦα刺激后，表面ＩＣＡＭ－１表达明显升高（Ｐ＜０．０１），转染细胞加ＴＮＦα刺激后表面ＩＣＡＭ－１表达无明显升高（Ｐ＞０．０５），转染细胞表面ＩＣＡＭ－１表达低于正常细胞（Ｐ＜０．０５）。结论：ＩＣＡＭ－１反义ＤＮＡ体外可抑制细胞ＩＣＡＭ－１表达。
Objective: To investigate the inhibitory effect of ICAM-1 antisense DNA on ICAM-1 expression in endothelial cells. Methods: The ICAM-1 antisense DNA vector was constructed and transfected into endothelial cells by liposome. The expression of ICAM-1 on the surface of transfected cells was detected by flow cytometry. Results: The restriction endonuclease digestion proved that the 1.4kb ICAM-1 DNA fragment was reversely ligated into the pcDNA3 expression vector. The results of flow cytometry showed that ICAM-1 expression was significantly increased in normal cells stimulated with TNFα (P <0. 01). The expression of ICAM-1 on the transfected cells plus TNFα was not significantly increased (P> 0.05). The expression of ICAM-1 on the transfected cells was lower than that on the normal cells (P <0.05). Conclusion: ICAM-1 antisense DNA can inhibit ICAM-1 expression in vitro.