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幽门螺杆菌（ＨＰ）是一种革兰氏阴性菌，已经证明ＨＰ与一些胃炎和消化性溃疡有较密切的关系。ＨＰ也是胃窦炎最常见的病因之一，并与远端胃癌和低度恶性胃淋巴瘤的发生有关。所以，越来越引起人们的重视。在临床病理工作中，胃粘膜活检不要仅局限于胃粘膜本身的病变检查，还需要进一步检查有无ＨＰ感染。ＨＰ是一种较弱的噬苏木素菌，对ＨＥ染色着色较淡，在观察时有一定的困难。为了明确有无ＨＰ感染常借助于特殊染色。用于ＨＰ特殊染色的方法有Ｇｉｅｍ－ｓａ染色法和银染色法等。我们采用改良的微波Ｇｉｅｍｓａ染色法，即在原Ｇｉｅｍｓａ染色法的基础上，加入了表面活性剂ＴｒｉｔｏｎＸ－１００，在染色过程中经微波炉加温处理。应用改良的Ｇｉｅｍｓａ染色法，可缩短染色时间。染色结果表明，胃粘膜组织和ＨＰ着色良好，细微结构清楚，结果明确。微波Ｇｉｅｍｓａ染色法用于胃粘膜石蜡切片染色，具有常规ＨＥ染色和特殊染色的双重效果，能同时清楚地显示出胃粘膜组织和ＨＰ的结构。
Helicobacter pylori (HP) is a Gram-negative bacterium that has been shown to have a close relationship with some gastritis and peptic ulcers. HP is also one of the most common causes of gastric gastritis and is associated with the development of distal gastric cancer and low-grade gastric lymphoma. Therefore, people pay more and more attention. In clinical pathology, gastric mucosal biopsy should not only be limited to the gastric mucosa lesions, but also need to further check for HP infection. HP is a weaker haematoxylin-producing bacterium that stains HE stained lighter and has some difficulty in observation. In order to clear whether HP infection often use special staining. For the special HP staining methods Giem-sa staining and silver staining and so on. We use a modified microwave Giemsa staining method, that is, based on the original Giemsa staining method, the surfactant TritonX-100 was added and heated in a microwave oven during the dyeing process. Application of improved Giemsa staining, can shorten the staining time. The staining results showed that the gastric mucosa and HP were well colored, the microstructure was clear and the result was clear. Microwave Giemsa staining for gastric mucosal paraffin sections with conventional HE staining and special staining of the dual effects can clearly show the structure of gastric mucosa and HP.