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报道了新型增强剂四苯硼钠对过氧化物酶催化鲁米诺-过氧化氢发光反应的增强作用,建立了流动注射化学发光测定或辣过氧化物酶(HRP)的新体系。用该体系测定HRP线性范围为1.0×10-12×1.2×10-13mol/L;检测限为0.6×10-13mol/L。对0.6×10-13mmol/L的 HRP进行11次平行测定,相对标准偏差为 1. 5%。
A new enhancer, tetraphenylboron, was reported to enhance the luminol peroxidase-catalyzed luminol-H2O2 luminescence reaction and to establish a new flow-injection chemiluminescence assay or spicy peroxidase (HRP) system. The linear range of HRP was 1.0 × 10-12 × 1.2 × 10-13mol / L. The detection limit was 0.6 × 10-13mol / L. 11 parallel determinations of 0.6 × 10-13 mmol / L HRP showed a relative standard deviation of 1. 5%.