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目的:观察严重烧伤大鼠肠黏膜zonula occludens-1(ZO-1)mRNA的表达和细菌易位,探讨严重烧伤时肠黏膜屏障功能的改变。方法:将50只大鼠随机分为对照组(n=20)和烧伤组(n=30)。对照组大鼠不作任何处理;烧伤组大鼠于背部造成30%总体表面积(TBSA)Ⅲ度烫伤(以下称烧伤)创面,伤后立即经腹腔补充乳酸林格液40 ml/kg。大鼠烧伤后24、48、72和96 h,取肠系膜淋巴结、肝、脾组织,匀浆后行细菌培养。采用逆转录-聚合酶链反应(RT-PCR)法检测空肠组织中ZO-1 mRNA表达水平。光镜下观察空肠黏膜组织的病理形态学变化。结果:在伤后72 h,肠系膜淋巴结、肝、脾组织匀浆细菌易位量烧伤组明显高于对照组;ZO-1 mRNA的表达水平对照组为0.854±0.0132,烧伤组为0.487±0.0153;空肠黏膜组织病理形态学观察,烧伤组肠黏膜绒毛数量减少,排列紊乱,绒毛顶端上皮细胞出现坏死和脱落。结论:严重烧伤大鼠早期存在肠黏膜屏障功能损伤,肠上皮细胞间连接增宽,其可能机制与肠上皮ZO-1 mRNA的表达降低有关。
OBJECTIVE: To observe the expression of zonula occludens-1 (ZO-1) mRNA and bacterial translocation in severely burned rats and to explore the changes of intestinal mucosal barrier function in severe burn rats. Methods: Fifty rats were randomly divided into control group (n = 20) and burn group (n = 30). The rats in the control group were given 30% TBSA burns (wounds) on the back, and the rats in the burn group were given lactic acid Ringer’s solution 40 ml / kg intraperitoneally immediately after the injury. At 24, 48, 72 and 96 h after burn, mesenteric lymph nodes, liver and spleen were harvested, and the cells were cultured after homogenization. The expression of ZO-1 mRNA in jejunum was detected by reverse transcription-polymerase chain reaction (RT-PCR). The pathological changes of jejunum mucosa were observed under light microscope. Results: Bacterial translocation in mesenteric lymph nodes, liver and spleen homogenates was significantly higher than that in control group at 72 h after injury. The expression of ZO-1 mRNA in control group was 0.854 ± 0.0132 and in burn group was 0.487 ± 0.0153. Jejunal mucosa histopathological observation, the number of intestinal mucosa villi in the burn group decreased, disordered, the top of the villous epithelial cells necrosis and fall off. CONCLUSIONS: In the early stage of severe burns, the intestinal mucosal barrier function is impaired and the intestinal epithelial connections are broadened. The possible mechanism is related to the decreased expression of ZO-1 mRNA in the gut epithelium.