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摘 要：对建立猪和牛的胚胎干细胞系（Embryonic stem cells，ESCs）的条件进行了摸索和优化，建立了良好的胚胎培养体系、内细胞团分离方法和饲养层的选择；另外分别建立猪和牛的诱导性多能性干细胞系（induced pluripotent stem cells，iPS），并分别对其进行了鉴定；对目前的培养体系进行了优化提高了iPS的效率和发育潜能；尝试以iPS为供体细胞进行核移植，以期获得iPS来源的动物种群。
关键词：猪胚胎干细胞 诱导多能性干细胞 重编程
Science，Biejing； 4.China Agricultural University；5.Shanghai Institute of Biological Sciences）
Abstract：Exploration and optimization the conditions for establishment of pigs and cattle embryonic stem cell lines （ESCs） to establish a good embryo culture system， the inner cell mass separation methods and choice of feeder layers. Established pigs and cattle induced pluripotent stem cells （iPSCs）， with respect to their identification； the current training system has been optimized to improve the efficiency and growth potential of iPSCs； try to obtain iPS-derived animal populations， by nuclear transfer that iPS cells as donor cells.
Key Words：Pig Embryonic Stem Cells；Induced Pluripotent Stem Cells；Reprogramming
阅读全文链接（需实名注册）：http：//www.nstrs.cn/xiangxiBG.aspx？id=14659&flag=1
关键词：猪胚胎干细胞 诱导多能性干细胞 重编程
Science，Biejing； 4.China Agricultural University；5.Shanghai Institute of Biological Sciences）
Abstract：Exploration and optimization the conditions for establishment of pigs and cattle embryonic stem cell lines （ESCs） to establish a good embryo culture system， the inner cell mass separation methods and choice of feeder layers. Established pigs and cattle induced pluripotent stem cells （iPSCs）， with respect to their identification； the current training system has been optimized to improve the efficiency and growth potential of iPSCs； try to obtain iPS-derived animal populations， by nuclear transfer that iPS cells as donor cells.
Key Words：Pig Embryonic Stem Cells；Induced Pluripotent Stem Cells；Reprogramming
阅读全文链接（需实名注册）：http：//www.nstrs.cn/xiangxiBG.aspx？id=14659&flag=1