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本文选用微量组胺测定技术，对中华硬蜱（Ｉｘｏｄｅｓｓｉｎｅｎｓｉｓ）雌性成虫叮咬初次和再次感染宿主（家兔）血液和局部皮肤组织中组胺含量进行测定。研究结果表明：中华硬蜱初次叮咬家兔后吸血量２３３．７８±４０．５３ｍｇ，宿主血液和局部组织中组胺含量第５天达高峰，分别为４０５．４４±１２０．２９ｎｇ／ｍｌ和３６２．３４±８４．７２ｎｇ／ｇ，而中华硬蜱再次叮咬感染组家兔吸血量为９６．４２±３６．２８ｍｇ，宿主血液和局部组织中组胺迅速升高，２４小时达高峰，分别为６４２．４３±１４６．４７ｎｇ／ｍｌ和１１５２．５３±２０３．９６ｎｇ／ｇ，与初次叮咬组胺含量相比有显著性差异（Ｐ＜０．０１）。提示组胺与宿主产生对蜱感染的获得性抵抗力有关。
In this paper, the histamine content in the blood of the female (Ixodessinensis) female adult bites initial and re-infected host (rabbit) blood and local skin tissue were measured by the method of microhistochemistry. The results showed that the amount of sucking blood of Chinese Ixodes sinensis was 237.78 ± 40.53mg after initial bite rabbits, and the histamine content peaked on the 5th day in the host blood and local tissues, which were 405.44 ± 120.29ng / ml and 362 .34 ± 84.72ng / g, while the Chinese ixodes tick bite infection again rabbit blood sucking amount of 96.42 ± 36.28mg, host blood and local histamine rapidly increased, reached the peak 24 hours, respectively, 642 .43 ± 146.47ng / ml and 1152.53 ± 203.96ng / g, respectively, which were significantly different from the initial bite histamine content (P <0.01). Suggesting that histamine is associated with acquired host resistance to tick infection.