目的探讨格列卫治疗慢性粒细胞白血病Ph染色体阴性后自体外周血干细胞移植的可行性。方法对2例成人慢性粒细胞白血病(CML)慢性期患者采用Ph染色体阳性细胞和间期荧光原位杂交(I-FISH)检查bcr/abl融合基因阳性细胞均≥90%,IFN-α治疗半年耐药,改用格列卫0.3~0.4 g/d分别治疗130 和168 d,期间3次复查Ph染色体和FISH检测bcr/abl融合基因均示阴性,此后应用阿糖胞苷2.0 g/d和足叶乙甙0.2 g/d分别静脉注射3 d,环磷酰胺1.0 g静脉注射1次。当白细胞<1.0×109/L时,应用G-CSF 300 μg/d,至白细胞>10×109/L时应用CS 3000 Plus分离外周血单个核细胞并用液氮保存待用。应用MiniMAC富集的CD34+细胞(纯度分别为83%和93%)的bcr/abl阳性率分别为11%和14%。动员完成后3~4周给予全身照射9 Gy,分2次照射,每天以环磷酰胺60 mg/kg·b.w.和足叶乙甙300 mg分别静脉注射2 d,静脉输注液氮保存的外周血干细胞,单个核细胞分别为4.17和3.9×108/kg·b.w.、CD34+细胞为4.89和4.8×106/kg·b.w.,移植-1~14 d应用CsA联合IL-2诱导移植物抗宿主病(GVHD)。结果移植后中性粒细胞绝对值>0.5×109/L平均需要11d,血小板>20×109/L平均需要20 d,无GVHD样表现。随访观察120 d和300 d,患者血液学持续缓解,但I-FISH检测骨髓细胞bcr/abl融合基因阳性 Objective To investigate the feasibility of glioma treatment of chronic myeloid leukemia Ph chromosome-negative autologous peripheral blood stem cell transplantation. Methods The positive cells of bcr / abl fusion gene were detected by Ph chromosome-positive cells and interphase fluorescence in situ hybridization (I-FISH) in two chronic adult patients with chronic myelogenous leukemia (CML) Resistant to Gleevec 0.3 to 0.4 g / d were treated for 130 and 168 d, respectively, three times during the Ph chromosome and FISH detection bcr / abl fusion gene showed negative, then application of cytarabine 2.0 g / d and Etoposide 0.2 g / d were intravenously injected for 3 days, cyclophosphamide 1.0 g intravenously once. Peripheral blood mononuclear cells (PBMCs) were isolated and pre-treated with liquid nitrogen (CS 3000 Plus) at a G-CSF concentration of 300 μg / day to leukocytes> 10 × 109 cells / L when leukocytes were less than 1.0 × 109 cells / The bcr / abl positive rates of MiniMAC-enriched CD34 + cells (83% and 93% purity, respectively) were 11% and 14%, respectively. Three to four weeks after the completion of the mobilization, whole-body irradiation was given at 9 Gy in 2 doses, and intravenous infusion of 60 mg / kg · bw cyclophosphamide and 300 mg of etoposide for 2 days each day was performed intravenously The numbers of stem cells, mononuclear cells were 4.17 and 3.9 × 108 / kg · bw respectively, while those of CD34 + cells were 4.89 and 4.8 × 106 / kg · bw respectively. CsA and IL-2 were used to induce graft-versus-host disease GVHD). Results The absolute value of neutrophil transplantation> 0.5 × 109 / L after transplantation was 11d on average, and the average platelet count> 20 × 109 / L needed 20 days on average, showing no GVHD. Follow-up observation of 120 d and 300 d, hematology continued to ease, but I-FISH detection of bone marrow cells bcr / abl fusion gene positive