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目的：研究宫颈癌患者的Ｐ５３基因蛋白表达和细胞增殖活性。方法：对５６例正常宫颈粘膜、宫颈上皮内瘤变、宫颈浸润性鳞癌用免疫组化ＡＢＣ法和图像分析法检测Ｐ５３蛋白、ＰＣＮＡ和ＤＮＡ含量。结果：Ｐ５３表达阳性率、ＰＣＮＡ细胞增殖指数和ＤＮＡ含量随癌组织分级依次增高。结论：Ｐ５３含量和细胞活性增高与宫颈粘膜癌变发生及恶性程度有关；ＰＣＮＡ增殖指数、ＤＮＡ含量和多倍体细胞的出现，对早期宫颈癌的诊断有一定意义；Ｐ５３蛋白在宫颈癌发生中不是一个早期事件。
Objective: To study the P53 gene protein expression and cell proliferation activity in cervical cancer patients. Methods: The levels of P53 protein, PCNA and DNA in 56 cases of normal cervical mucosa, cervical intraepithelial neoplasia, and cervical invasive squamous cell carcinoma were detected by immunohistochemical ABC method and image analysis. Results: The positive rate of P53 expression, cell proliferation index and DNA content of PCNA increased with the grade of cancer tissue. Conclusion: The increase of P53 content and cell activity correlates with the carcinogenesis and malignancy of cervical mucous membrane. The proliferation index, DNA content and the appearance of polyploid cells in PCNA are of significance in the diagnosis of early cervical cancer. P53 protein is not in the occurrence of cervical cancer. An early event.