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为了应用寡聚核苷酸基因表达谱芯片研究 9对白血病患者 /同胞供受者对之间的基因表达谱差异以识别主要的疾病相关基因 ,将 16 3条白血病 /肿瘤发病相关基因组群列入芯片设计 ,合成寡核苷酸探针 ,用点样仪点片制成基因芯片。提取病初白血病患者及其供者的骨髓 /外周血标本或其相对应的健康同胞供者经G CSF动员后并经CS 30 0 0细胞分离机采集的浓集的 9份外周血造血干细胞的总RNA ;分别逆转录并进行寡核苷酸探针杂交。结果表明 :与其相应的正常供者配对比较 ,发现 4例ALL患者中存在 6条显著差异表达基因 ,其中上调表达基因 5条(RIZ ,STK 1,T cellleukemia/lymphoma 1A ,Cbp/ p30 0 ,Op18) ,下调表达 1条 (hematopoieticproteoglycancorepro tein) ;5例AML M4和AML M5患者中亦存在 7条显著差异表达基因 ,其中上调表达 6条 (STAT5B ,ligandp6 2fortheLckSH2 ,CST3,LTC4S ,myeloidleukemiafactor 2 ,epb72 ) ,下调表达 1条 (CCR5 )。结论 :选择有高度遗传关联的兄弟姐妹供受者对作为差异研究比照对象 ,利用寡聚核苷酸基因芯片技术进行疾病基因的筛查 ,筛查出了 13条主要异常基因 ;进一步确认了上述基因在白血病分子发病机制中的关键性作用。
In order to identify differences in gene expression profiles among nine pairs of leukemia / sib recipient pairs using oligonucleotide gene expression profiling to identify major disease-related genes, 163 leukemia / oncogenesis-related genomic populations were included Chip design, synthesis of oligonucleotide probes, spotting chip made of gene chip. Bone marrow / peripheral blood samples of patients with early leukemia and their donors or their corresponding healthy sibling donor were collected after mobilization by G CSF and concentrated by a CS 30 0 0 cytocentrifuge Total RNA was reverse transcribed and subjected to oligonucleotide probe hybridization. The results showed that there were 6 significant differentially expressed genes in 4 ALL patients, of which 5 were up-regulated (RIZ, STK 1, T cellleukemia / lymphoma 1A, Cbp / p30 0, Op18 ) And down-regulated expression (hematopoietic proteoglycancorepro tein). There were also 7 differentially expressed genes in 5 AML M4 and AML M5 patients, of which 6 were up-regulated (STAT5B, ligandp62fortheLckSH2, CST3, LTC4S and myeloidleukemiafactor 2, epb72) Down-regulated expression of 1 (CCR5). CONCLUSIONS: The sibling donors with highly inherited genetic markers were selected as the target of difference study. The gene of disease was screened by oligonucleotide microarray and 13 major abnormal genes were screened out. Genes play key roles in the pathogenesis of leukemia.