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以4份叶用莴苣栽培品种为材料，比较了激素组合、AgNO3及其浓度和不同外植体切段对不定芽形成的影响。结果表明，在本实验条件下ZT1mg／L和IAA1mg／L组合的出芽外植体百分率最高，平均为20．14％；培养基中添加AgNO3可明显提高植株再生频率，增加每个外植体的丛生芽数目，浓度以5mg／L为最好，具芽外植体百分率平均为44．90％；真叶的培养效果好于子叶，真叶柄的培养效果优于真叶其它部位，以真叶柄为外植体，在添加AgNO35mm／L和CH1000mg／L的培养基上获得了98．00％以上的出芽外植体百分率。
Four cultivars of leaf lettuce were used as materials to compare the effects of hormone combinations, AgNO3 and their concentrations and the different explants on the formation of adventitious buds. The results showed that the budding explants with the combination of ZT1mg / L and IAA1mg / L had the highest percentage of budding explants under the experimental conditions, with an average of 20.14%. Adding AgNO3 to the medium significantly increased the frequency of plant regeneration, The number of clustered buds was best at 5mg / L, and the average percentage of explants with buds was 44.90%. The culture effect of true leaves was better than that of cotyledons, the effect of true petiole was better than that of other leaves, As explants, more than 98.00% of budding explants were obtained on medium supplemented with AgNO35mm / L and CH1000mg / L.