【摘 要】
：
Background Retinal pigment epithelial (RPE) cell is a monolayer of multifunctional cells between the retina and the expression of inducible nitric oxide synthas
【机 构】
：
Department of Ophthalmology
论文部分内容阅读
Background Retinal pigment epithelial (RPE) cell is a monolayer of multifunctional cells between the retina and the expression of inducible nitric oxide synthase (iNOS) and complement 3 (C3) via Fas/FasL pathway in RPE cells and the values of puerarin as a therapeutic target for inhibiting the apoptosis of RPE cells.flow cytometry was used to determine the apoptosis of RPE cells. Immunohistochemistry and Western blotting were used to determine Fas/FasL signal transduction. Gene array analysis, reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to determine the expression of iNOS mRNA and iNOS protein in RPE cells.Results There were minor expression of NT, C3, Fas/FasL and iNOS mRNA in control group, and strong expression of down-regulated in puerarin group (P＜0.001).damage on RPE cells. The antagonizing mechanism of puerarin may be related to its inhibitory to the expression of iNOS puerarin may be an useful therapeutic agent against apoptosis of RPE cells.
其他文献
Background Nucleophosmin plays a critical role in embryonic development. This study aimed to examine the expression pattern of nucleophosmin in glandular epithe
本文以AK模型为理论分析我国1978～2011年间经济发展的动力，并近似估计了改革开放后我国的储蓄率与折旧率，发现其结果和我国的实际比较接近，说明我国经济发展的动力是高储蓄率，同时
Background Paraquat (PQ), an effective and widely used herbicide, has been proven to be safe when appropriately applied to eliminate weeds. However, PQ poisonin
A method was established using hollow fiber-liquid phase microextraction(HF-LPME) followed by high performance liquid chromatography(HPLC) to determine the conc
Migration of metallic foreign body into the cerebral circulation is rarely seen. Most of the cases reported were due to gunshot wounds and shotgun wounds to the
Background Traumatic brain injury (TBl) often causes cognitive deficits and remote symptomatic epilepsy.Hippocampal regional excitability is associated with the
Background Prevalence of inadequate glycaemic control among patients with type 2 diabetes mellitus (T2DM)remains high. We assessed glycaemic control in the real
Background Random flap is one kind of the most widely used skin flaps in reconstructive surgery; however, partial necrosis of its distal end remains a significa
Background Simultaneous pancreas-kidney transplantation (SPKT) is the best treatment option for diabetic patients with advanced chronic renal failure. The curre