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目的 采用基因芯片技术来筛选新基因AngRem104的功能相关基因,为进一步的基因功能研究提供线索。方法 建立新基因AngRem104的正义和反义表达的系膜细胞模型,应用含有4000个人类基因的cDNA表达谱芯片,对过量表达AngRem104基因和抑制表达AngRem104基因的人肾小球系膜细胞RNA表达进行检测。部分基因的差异表达由RT-PCR方法来验证。结果 筛选出的差异表达基因共96个,其中94个基因上调表达,2个基因下调表达。差异表达的基因涉及到细胞外基质和受体蛋白、细胞信号传导相关基因、DNA结合及转录相关蛋白、免疫相关蛋白、细胞骨架和动力蛋白和细胞合成及代谢相关蛋白等。特别是纤连蛋白(FN)及整合素β1(integrin β1)表达明显上调。RT-PCR的方法也证实了AngRem104与FN表达的相关性。结论 应用作为研究基因功能有效手段的基因表达谱芯片技术来筛选新基因AngRem104的功能相关基因,发现AngRem104与FN的表达有关,为其功能研究提供了重要线索。
Objective To screen gene-related genes of AngRem104 by gene chip technology and provide clues for further study of gene function. Methods We established a model of mesangial cells with sense and antisense expression of a novel gene AngRem104. The gene expression profile of AngRem104 and the mRNA expression of AngRem104 in human glomerular mesangial cells were detected by cDNA microarray with 4000 human genes Detection. The differential expression of some genes was verified by RT-PCR method. Results A total of 96 differentially expressed genes were screened, of which 94 were up-regulated and two were down-regulated. Differentially expressed genes involved in extracellular matrix and receptor proteins, cell signal transduction related genes, DNA binding and transcription related proteins, immune-related proteins, cytoskeleton and motor protein and cell synthesis and metabolism-related proteins. In particular, fibronectin (FN) and integrin β1 (integrin β1) expression was significantly increased. The method of RT-PCR also confirmed the correlation between AngRem104 and FN expression. Conclusion Gene expression microarray technology, as an effective means of studying gene function, was used to screen the function related genes of AngRem104. It was found that AngRem104 was related to the expression of FN, which provided important clues to its function study.