淋巴结结构比较复杂,在常规制片中难度较大,如切片过厚,染色对比度差,组织易碎,各种试剂难以渗透,故而造成切片质量较差,给诊断带来困难甚至误诊[1]。造成以上切片不良的原因是多方面的,其中取材、固定、脱水、透明、浸蜡、染色等任何一步处理不当均可导致切片不良[2]。本文就 Lymph node structure is more complex, more difficult in the conventional production, such as sliced ​​too thick, poor contrast, tissue fragile, difficult to penetrate a variety of reagents, resulting in poor slice quality, to the diagnosis of difficult or even misdiagnosed [1] . There are many reasons for the poor slicing. Among them, improper slicing can be caused by improper handling such as drawing, fixing, dehydration, transparency, waxing and dyeing, etc. [2]. This article is