A membrane composed of an alginate (ALG) layer and a chitosan (CHS) layer with sustained antimicrobial efficacy was prepared. Ciprofloxacin HCI (CIP) was incorporated into the ALG layer. Morphological feature of the composite membrane was analyzed by scanning electron microscopy (SEM). Water uptake capacity, in vitro drug release, and in vitro antimicrobial activity were evaluated. The composite membrane exhibited perfect binding characteristic between the two layers. The water uptake capacity of all the membranes was above 800%. The CIP could release from the composite membranes for 48 h. The membrane could control the bacterial growth persistently. The results suggested that this CHS/ALG composite membrane incorporated with CIP had the potential for wound dressing application.