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目的:探讨ALA-PDT结合hTGF-β1基因转染对结肠癌细胞体外增殖的抑制作用。方法:以腺病毒为载体将hTGF-β1基因导入人结肠癌细胞株8W480后,行PDT处理,应用免疫组化法检测TGF-β1的表达情况。通过MTT法检测转染后细胞增殖的抑制情况,应用流式细胞技术检测细胞周期的变化。结果:转染hTGF-β1基因后,TGF-β1蛋白表达明显增强。与单纯行PDT相比,G0/S周期阻滞作用更为明显。对SW480的增殖抑制效果明显提高(p<0.05)。结论:转染hTGF-β1基因可明显增强ALA-PDT对体外培养的结肠癌细胞SW480的增殖抑制作用。
Objective: To investigate the inhibitory effect of ALA-PDT combined with hTGF-β1 gene transfection on colon cancer cell proliferation in vitro. METHODS: Adenovirus was used as the vector to introduce hTGF-β1 gene into human colon cancer cell line 8W480, followed by PDT. The expression of TGF-β1 was detected by immunohistochemistry. The inhibition of cell proliferation after transfection was detected by MTT assay, and cell cycle changes were detected by flow cytometry. RESULTS: After transfected with hTGF-β1 gene, the expression of TGF-β1 protein was significantly increased. Compared with PDT alone, G0/S cycle arrest is more pronounced. The inhibitory effect of SW480 on proliferation was significantly increased (p<0.05). Conclusion: Transfection of hTGF-β1 gene can significantly enhance the inhibitory effect of ALA-PDT on colon cancer cell SW480 in vitro.