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关于姐妹染色单体互换(SCE)的研究多取材于外周血,其次为骨髓与成纤维细胞,引用其他组织尚不多见。而采用羊水细胞方面,由于过去对这一工作重视不够,迄今尚缺少可借鉴的文献。虽然不同组织的SCE 制备原则是一致的,但具体步骤与注意点各不相同,为了便于这一工作的开展,特将其培养注意事项及染色方法的具体步骤介绍于下.实验方法一、培养与制片羊水细胞培养制备SCE,曾分别采用Ham F_(10),Ham F_(12),McCoy 5A 等培基,均取得良好的效果。制备上述培基时,一般均依规定量将粉剂溶解于三蒸水中,然后经G_6玻璃滤器过滤灭菌,再与小牛血清以4:1相互混合配成培养液,其pH 约为6.7—6.9,每100毫升培养液中加入青、链霉素或卡那霉素10000单位.
On the sister chromatid exchange (SCE) of the research is more based on peripheral blood, followed by bone marrow and fibroblasts, citing other organizations is still rare. The use of amniotic fluid cells, because the past paid insufficient attention to this work, so far there is no available reference. Although the principles of SCE preparation are the same in different organizations, the specific steps and points of attention are different. In order to facilitate this work, the specific steps of training precautions and staining methods are introduced below. And preparation of amniotic fluid cell culture preparation of SCE, had Ham F_ (10), Ham F_ (12), McCoy 5A and other training base, have achieved good results. Preparation of the above-mentioned Pei-based, usually according to the required amount of powder will be dissolved in three steaming water, and then sterilized by G_6 glass filter, and then mixed with bovine serum 4: 1 formulated into a culture medium, the pH of about 6.7- 6.9, add 100 units of cyan, streptomycin or kanamycin per 100 ml of culture medium.