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目的了解PPAR-γ偶联的信号通路在大鼠肝再生(LR)中作用。方法用搜集网站资料和查阅相关论文等方法获得上述通路相关基因,用RatGenome 230 2.0芯片检测它们在大鼠肝再生中表达情况,用真手术与手术对照比较方法确定肝再生相关基因。结果初步证实上述基因中64个基因与肝再生相关。肝再生启动、G0/G1过渡、细胞增殖、细胞分化和组织结构功能重建等4个阶段起始表达的基因数为28、4、34和2,基因总表达次数为72、41、247和90,表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共分为11种表达方式,表明肝再生中这些基因表达变化多样和复杂。结论PPAR-γ偶联的信号通路在肝再生早期、前期和后期促进糖元合成;在整个肝再生中抑制炎症反应,促进细胞增殖和迁移。
Objective To understand the role of PPAR-γ-coupled signaling pathway in rat liver regeneration (LR). Methods The relevant genes were obtained by means of collecting site information and consulting relevant papers. Rat Genome 230 2.0 was used to detect the expression of these pathways in rat liver regeneration. The relative genes of liver regeneration were identified by comparing the true operation and operation control method. The results initially confirmed that 64 of the above genes were associated with liver regeneration. The number of genes initially expressed in four stages of liver regeneration initiation, G0 / G1 transition, cell proliferation, cell differentiation and functional reconstruction of tissue structure was 28,4,34 and 2, and the total number of gene expression was 72,41,247 and 90 , Indicating that the relevant genes are mainly expressed in the initiation phase of liver regeneration and play a role in different stages. They are divided into 11 types of expression, indicating that these genes in liver regeneration vary widely and complicatedly. Conclusion The PPAR-γ-coupled signaling pathway promotes glycogen synthesis during the early, early and late stages of liver regeneration, inhibits the inflammatory response and promotes cell proliferation and migration throughout liver regeneration.