应用体外骨髓基质细胞贴壁培养的方法,观察了小鼠骨髓贴壁细胞的形态及其对造血的调控作用。结果表明,两类大小、形态、吞噬功能及组化染色有所差异的基质细胞群体分别具有巨噬细胞和成纤维细胞样特征。贴壁细胞对粒—单系祖细胞的增殖表现为明显的刺激作用,而对多能造血干细胞无明显调控。提示骨髓基质细胞在造血祖细胞阶段的分化增殖过程中具有一定作用。 In vitro bone marrow stromal cells adherent culture method was observed mouse bone marrow adherent cells morphology and hematopoietic regulation. The results showed that two groups of stromal cells with different size, morphology, phagocytosis and histochemical staining had macrophages and fibroblast-like features, respectively. Adherent cells on the granulocyte - monocyte progenitor cell proliferation showed a significant stimulus, and pluripotent hematopoietic stem cells did not significantly regulate. Tip bone marrow stromal cells in the hematopoietic progenitor cell differentiation and proliferation process has a role.