人工转录因子是通过模拟天然转录因子的结构,由人为设计的DNA结合结构域和功能结构域组成。人工转录因子的构建包括DNA结合结构域和功能结构域的构建,通过体外设计筛选或模建,然后进入体内与特异DNA序列结合调控目的基因的表达;目前有关人工转录因子的应用研究主要集中在人类疾病的治疗,药物发现和生产,农业生物技术及基因功能的研究等方面。 Artificial transcription factors are composed of human-engineered DNA-binding domains and functional domains by mimicking the structure of native transcription factors. The construction of artificial transcription factors includes the construction of DNA binding domain and functional domain, and the in vitro design of screening or modeling, and then into the body with specific DNA sequences to regulate the expression of the target gene; the current application of artificial transcription factors mainly concentrated in Treatment of human diseases, drug discovery and production, agricultural biotechnology and gene function research.