从土壤中分离、筛选出一株同化乙醇能力强的微生物,将其固定化,制备成微生物膜,并与氧电极结合,组成微生物乙醇传感器。研究了温度、pH等实验条件的影响。测得传感器对乙醇的线性响应范围为0.3～12.0mg/L,响应时间为2～5mm,三个月内测定700余次,其灵敏度基本不变。将传感器用于含醇饮料中乙醇含量的测定,获得了满意结果。 Separated from the soil, screened a strong ethanol assimilation ability of microorganisms, immobilized, prepared into a microbial membrane, and combined with the oxygen electrode to form a microbial ethanol sensor. The effects of experimental conditions such as temperature and pH were studied. The linear response range of the sensor to ethanol was 0.3 ~ 12.0mg / L, the response time was 2 ~ 5mm, and the sensitivity was almost unchanged after 700 times within three months. The sensor was used to determine the content of ethanol in alcoholic beverages, and satisfactory results were obtained.