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用Mayahara改良法检出质膜Na~+-K~+-ATP酶活性,以对-硝基苯酚磷酸盐(P-NPP)为底物,检出K~+依赖、硅巴因敏感,参与Na~+-K~+-ATP酶系统中第二步脱磷酸作用的对-硝基苯酚磷酸酶(K~+-NPP酶),用以反映Na~+-K~+-ATP酶活性的存在部位。实验证实此方法特异性强,非特异沉淀干扰少,为研究Na~+-K~+-ATP酶亚细胞定位的一种好方法。应用此法观察到过量异丙基肾上腺素(ISO)能抑制心肌细胞质膜Na~+-K~+-ATP酶活性;钙拮抗剂异搏定与ISO同时应用能保护Na~+-K~+-ATP酶活性。文中分析了Na~+-K~+-ATP酶活性与Ca~(2+)浓度变化的关系。
The plasma membrane Na ~ + -K ~ + -ATPase activity was detected by Mayahara modified method, K ~ + -dependent and p-NPP were detected as substrates, The second step of dephosphorylation of p-nitrophenol phosphatase (K ~ + -NPPase) in Na ~ + -K ~ + -ATPase system is to reflect the activity of Na ~ + -K ~ + -ATPase There are parts. Experiments show that this method is specific and non-specific precipitation less interference, for the study Na ~ + -K ~ + -ATP enzyme subcellular localization of a good way. Using this method, it was observed that excess isoproterenol (ISO) could inhibit the plasma membrane Na ~ + -K ~ + -ATPase activity of cardiomyocytes; The combination of calcium antagonist verapamil and ISO could protect Na ~ + -K ~ -ATPase activity. The relationship between Na ~ + -K ~ + -ATPase activity and Ca ~ (2+) concentration was analyzed.