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目的：研究蒿甲醚（Ａｒｔ）对日本血吸虫３磷酸甘油醛脱氢酶（ＧＡＰＤＨ）、磷酸甘油酸激酶（ＰＧＫ）和丙酮酸激酶（ＰＫ）的影响．方法：小鼠感染血吸虫尾蚴３２－３８ｄ后ｉｇＡｒｔ１００－３００ｍｇ·ｋｇ－１，２４－７２ｈ后取虫测定上述３种酶和乳酸含量．结果：小鼠ｉｇＡｒｔ３００ｍｇ·ｋｇ－１后２４－４８ｈ，血吸虫♀、♂虫的ＰＧＫ和ＰＫ活力被抑制２７％－４８％；♀虫的ＧＡＰＤＨ对Ａｒｔ亦较敏感，♂虫则否．给药后７２ｈ，♀虫乳酸含量降低７２％，♂虫的降低４９％．结论：Ａｒｔ对血吸虫的ＰＧＫ和ＰＫ活力有明显抑制作用，♀虫的ＧＡＰＤＨ对Ａｒｔ亦较敏感，♀虫乳酸含量降低较♂虫明显
Objective: To study the effects of artemether (Art) on glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycerate kinase (PGK) and pyruvate kinase (PK) in Schistosoma japonicum. METHODS: The mice were infected with igArt after 30-38 days of igArter igArt100-300mg·kg-1. After 24 to 72h, the above three enzymes and lactic acid content were measured. Results: The PGK and PK activities of schistosomiasis and aphids were inhibited from 27% to 48% after 24-48h of mice igArt300mg·kg-1. The GAPDH of aphids was also sensitive to Art and the aphids were not. 72h after administration, the lactic acid content of the aphids decreased by 72% and the aphid decreased by 49%. Conclusion: Art has a significant inhibitory effect on the PGK and PK activity of Schistosoma japonicum. GAPDH of aphids is also sensitive to Art. Aphid lactic acid content is lower than that of aphids.