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目的:观察反流性食管炎(RE)、Barrett食管(BE)、食管腺癌(EA)中食管上皮细胞的P38表达与细胞增殖凋亡的变化特点。方法:胃镜下取食管黏膜活检,免疫组化法和免疫印迹法测定RE组、BE组、EA组及正常对照组的P38和Ki-67的蛋白表达,TUNEL法测定细胞凋亡。结果:免疫组化法测定正常对照组、RE组、BE组、EA组的Ki-67表达率分别为10%、55%、60%、83.3%。TUNEL法测定正常对照组、RE组、BE组、EA组的细胞凋亡指数分别为(1.51±1.06)%、(8.12±1.55)%、(7.25±1.87)%、(3.63±1.70)%。免疫印迹法测定RE组、BE组的磷酸化P38蛋白吸光度比值分别为0.09±0.03、0.11±0.04,EA组和正常对照组无磷酸化P38蛋白表达。结论:与食管腺癌组相比,反流性食管炎、Barrett食管的细胞增殖率下降,细胞凋亡指数增加,伴随着磷酸化P38蛋白的表达增加,提示P38信号通路可能参与了反流性食管炎、Barrett食管发病过程中细胞增殖凋亡的调控。
Objective: To observe the changes of P38 expression and cell proliferation and apoptosis in esophageal epithelial cells in reflux esophagitis (RE), Barrett’s esophagus (BE) and esophageal adenocarcinoma (EA). Methods: The esophageal mucosa biopsies were taken under gastroscope. The protein expressions of P38 and Ki-67 in RE group, BE group, EA group and normal control group were determined by immunohistochemistry and Western blot. The apoptosis was detected by TUNEL. Results: The expression of Ki-67 in normal control group, RE group, BE group and EA group were 10%, 55%, 60% and 83.3% respectively by immunohistochemistry. TUNEL assay showed that the apoptotic index of normal control group, RE group, BE group and EA group were (1.51 ± 1.06)%, (8.12 ± 1.55)%, (7.25 ± 1.87)%, (3.63 ± 1.70)%, respectively. The absorbance ratios of phosphorylated P38 protein in RE group and BE group were 0.09 ± 0.03 and 0.11 ± 0.04 respectively by immunoblotting, and the phosphorylation of P38 protein in EA group and normal control group was no significant difference. Conclusion: Compared with esophageal adenocarcinoma group, reflux esophagitis, Barrett’s esophagus cell proliferation rate decreased, apoptosis index increased, accompanied by increased phosphorylation of P38 protein, suggesting that P38 signaling pathway may be involved in the reflux Esophagitis, Regulation of Cell Proliferation and Apoptosis during the Barrett’s Esophagus.