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目的探讨Fe3O4磁性纳米微球磁感应对Wistar大鼠Walker-256肿瘤的治疗作用。方法荷瘤鼠随机分为NS组(注入生理盐水而不接受磁场处理)、MF组(注入磁流体而不接受磁场处理)、MFH1组(注入磁流体在交变磁场中治疗1次)、MFH2组(治疗2次)和MFH3组(治疗3次),共5组。治疗温度控制在50～55℃,分别观察肿瘤体积抑制率和生存期,以及肿瘤的病理改变。结果MFH2组和MFH3组的肿瘤在2周内有明显的肿瘤体积抑制作用,肿瘤体积抑制率分别为(65.0±3.5)%、(71.6±4.2)%。与NS组(35.5±5.6)d比较,MFH3组(41.9±6.5)d和MFH2组(42.1±5.0)d的生存期延长,差异有统计学意义(q=4.386,P<0.05;q=4.558,P<0.05);MFH1组(39.0±5.3)d和MF组(36.1±6.6)d的生存期差异无统计学意义(q=2.406,P>0.05)。NS、MF组肿瘤表面光滑,小灶性坏死区;癌细胞密度大,核仁明显,易见核分裂相。MFH2和MFH3组瘤内大片坏死残留的无结构红染物质,或空洞,部分胞核有固缩、碎裂及崩解。结论Fe3O4纳米磁流体介导的磁热疗能抑制大鼠Walker-256皮下肿瘤增殖。
Objective To investigate the therapeutic effect of Fe3O4 magnetic nanospheres magnetic induction on Walker-256 tumor in Wistar rats. Methods The tumor-bearing mice were randomly divided into NS group (injected with saline without magnetic field treatment), MF group (with magnetic fluid injection but without magnetic field treatment), MFH1 group (treated with magnetic fluid in alternating magnetic field), MFH2 Group (treatment 2 times) and MFH3 group (treatment 3 times), a total of 5 groups. The treatment temperature was controlled at 50 ~ 55 ℃, the tumor volume inhibition rate and survival time were observed, and the pathological changes of the tumor. Results The tumors in MFH2 and MFH3 groups showed significant inhibition of tumor volume within 2 weeks. The tumor volume inhibition rates were (65.0 ± 3.5)% and (71.6 ± 4.2)%, respectively. Compared with NS group (35.5 ± 5.6) d, MFI3 group (41.9 ± 6.5) d and MFH2 group (42.1 ± 5.0) d had longer survival time (q = 4.386, P <0.05; , P <0.05). There was no significant difference in the survival time between MFH1 group (39.0 ± 5.3) d and MF group (36.1 ± 6.6) d (q = 2.406, P> 0.05). NS, MF tumor surface smooth, small focal necrosis area; cancer cell density, nucleoli obvious, easy to see the mitotic phase. In the MFH2 and MFH3 tumors, large number of necrotic non-structural red dye remained in the tumor, or voids. Some nuclei were condensed, disintegrated and disintegrated. Conclusion Fe3O4 nanofluid-mediated magnetocaloric hyperthermia can inhibit the proliferation of Walker-256 subcutaneous tumor in rats.