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目的 探讨布地奈德(BUD)对哮喘大鼠气道重塑及支气管肺泡灌洗液(BALF)、肺组织中转化生长因子-β1(TGF-β1)的影响,为糖皮质激素在哮喘中的应用提供理论依据.方法 健康成年雄性Wistar大鼠60只,随机分为3组.正常对照组、哮喘模型组和BUD治疗组.每组20只.用卵蛋白(OVA)致敏和激发制成哮喘大鼠模型,治疗组应用BUD雾化吸入治疗2周(1 mg/kg,30 min/次,qod),对照组用生理盐水代替.造模完成后,处死大鼠,左肺行支气管肺泡灌洗.右肺行病理学检查.ELISA法检测BALF上清中TGF-β1的含量,免疫组化法检测肺组织中TGF-β1的表达,Masson染色观察肺组织胶原沉积情况,计算机图像分析仪评价呼吸性细支气管平滑肌厚度(μm)及上皮损伤程度评分等气道重塑指标.结果 造模后,模型组大鼠BALF上清液及肺组织中TGF-β1的表达较对照组增多(P<0.01),与气道重翅的指标呈显著正相关;治疗组大鼠BALF上清液及肺组织中TGF-β1的表达较模型组减少(P<0.05).气道黏膜上皮损害、气道平滑肌厚度、气道胶原蛋白的沉积较模型组明显减轻.结论 哮喘大鼠BALF及肺组织中TGF一β1的表达增多,加重了气道重塑;BUD可以在一定程度上干预哮喘气道重塑,可能是通过减少气道局部TGF-β1的表达来实现的.“,”Objectives To explore the effects of budesonide (BUD) on airway remodeling and the levels of transforming growth factor-β1 (TGF-β1) in bronchoalveolar lavage fluid (BALF) and its expression in lung tissue of asthmatic rats. To provide a theoretical basis for the intervention and treatment of asthma. Methods Sixty healthy adult male Wistar rats were randomly divided into 3 groups: control group, asthma model group and BUD treated group, 20 rats in each group. A rat asthma model was established by ovalbumin (OVA) challenging. BUD treated group was treated with inhaled BUD 1 mg/kg in 30 min per serving every other day for two weeks. After OVA challenge finished, the rats were anesthetized and sacrificed for BALF and lung tissue collection. The level of TGF-β1 in BALF was measured by enzyme-linked immunosorbent assay. TGF-β1 expression and collagen deposition in the lung tissue were tested with immunohistochemical determination and Masson stain respectively. The computer image analysis system was used for measuring respiratory bronchiole smooth muscle thickness (μm) and the extent of epithelial damage score and other indicators of airway remodeling. Results Compared with that in the control group, TGF-β1 in BALF and lung tissue of asthma model group increased and were correlated significantly with the indicators of airway remodeling (P < 0.01) ;while that of BUD treated group reduced statistically significant than model group (P < 0.05). Pathology examination on asthma rat airway epithelial tissue showed the thickness of airway smooth muscle, the airway deposition of collagen in BUD treated group reduced obviously than asthma model group. Conclusions The expression of TGF-β1 in the rat asthma airway worsens the airway remodeling. The effects of BUD on ameliorating the progression of airway remodeling may be partially made by reducing the expression of TGF-β1. (J Clin Pediatr,2010,28(2):173-177)