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目的 :观察慢性低氧环境下大鼠NOS、NO的含量 ,及肺动脉压和肺血管结构的变化 ;方法 :将Wistar大鼠从 2 2 60m的高度带到 3 780m处 ,饲养 3 0天后分别测试肺动脉压的变化 ,NOS、NO的含量 ,观察肺部血管结构的改变 ,并与同一海拔高度的适应动物高原鼠兔进行了比较 ;结果 :缺氧 3 0天的大鼠肺动脉压明显高于高原鼠兔 ,两组分别为3 .5 4kPa± 0 .1 6kPa和 1 .5 9kPa± 0 .0 8kPa,P <0 .0 1 ,NOS的活性及NO的含量低于高原鼠兔 ;两组肺小血管在直径基本相同的情况下 ,大鼠血管壁明显增厚 ,血管壁占管径的百分比为 2 8.40 % ,高原鼠兔肺小动脉壁较薄 ,血管壁占管径的百分比为 1 2 .42 % ,两组间比较P<0 .0 1。肺小动脉中膜厚度与肺动脉平均压呈正相关r=0 .68,r =0 .87;结论 :低氧环境下NOS及NO的代谢异常是缺氧性血管结构重建和肺动脉高压形成的重要因素
OBJECTIVE: To observe the changes of NOS, NO and pulmonary arterial pressure and pulmonary vascular structure in rats under chronic hypoxic environment.Methods: The Wistar rats were taken from the height of 2260m to the height of 3780m, and were tested separately for 30 days The changes of pulmonary artery pressure, NOS and NO contents were observed. The changes of vascular structure in the lungs were observed and compared with that of the plateau pikas at the same altitudinal altitude. Results: The pulmonary pressure of the rats exposed to hypoxia for 30 days was significantly higher than that of the plateau The rats in the two groups were 3.5kPa ± 0.1 6kPa and 1.59kPa ± 0.08kPa, P <0.01. The activity of NOS and the content of NO were lower than those in the plateau pika In the condition of the same diameter, the vessel wall of rat was thicker, the percentage of vessel wall to tube diameter was 2. 8.40%, the wall of pulmonary arteriole was thinner and the percentage of vessel wall to tube diameter was 12 .42%, P <0. 01 between the two groups. Pulmonary arterioles in the media thickness and pulmonary artery mean pressure was positively correlated r = 0.68, r = 0.87; Conclusion: hypoxia environment NOS and NO metabolism abnormal hypoxia vascular remodeling and formation of pulmonary hypertension is an important factor