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选用两种典型的稀土上转换发光纳米粒子,即表面包覆氨基带正电的Na YF4:Yb3+,Er3+纳米粒子(HINPs)和包裹巯基带负电的Na YF4:Yb3+,Er3+纳米粒子(HMNPs),探究这两种粒子与细胞相互作用的机制。虽然HINPs和HMNPs的表面电性不同,但是理化性质相近、荧光发光强度相差不大,因此不会造成显著的成像差异。通过使用共聚焦成像和流式细胞仪进行检测,对比三种培养环境(正常培养条件、抑制膜蛋白活性培养条件和阻碍细胞内消耗能量运输过程的培养条件)的实验结果,发现HINPs和HMNPs的跨膜是一种消耗能量的受体介导的胞吞运输过程。另外,细胞对HINPs的摄取量要远大于对HMNPs的摄取量,说明带正电的粒子更容易和带负电的细胞膜相结合并进入细胞。
Two kinds of rare-earth up-conversion luminescent nanoparticles were selected: NaYF4: Yb3 +, Er3 + nanoparticles (HINPs) coated with amino positively charged surface and NaYF4: Yb3 + and Er3 + nanoparticles (HMNPs) Explore the mechanisms by which these two particles interact with the cells. Although the surface electrical properties of HINPs and HMNPs are different, their physical and chemical properties are similar and their fluorescence intensities are similar to each other, thus, no significant imaging difference will be caused. By using confocal imaging and flow cytometry, we compared the experimental conditions of three culture environments (normal culture conditions, inhibition of membrane protein activity culture conditions, and hindrance of intracellular energy consumption transport), and found that HINPs and HMNPs Transmembrane is an energy-consuming receptor-mediated process of endocytosis. In addition, the uptake of HINPs by cells was much greater than that of HMNPs, indicating that positively charged particles are more likely to associate with negatively charged cell membranes and enter cells.