1.培养基配制:于100毫升注射用水瓶内抽取10毫升注射用水弃去,注入 Hamk’s液甲原液5毫升,0.5%酚红0.1毫升,高压灭菌15磅30分钟,取出注入煮沸消毒 Hamk’s 原乙液5毫升,PHA 50毫克,“PHA 用广州医药工业研究所10毫克注射用针剂”,青霉素“2万单位/毫升”0.6毫升,链霉素“0.5克”0.25毫升,肝素“500单位/毫升”3毫升。必要时 1. Medium Preparation: 10 ml of water for injection into 100 ml water for injection was discarded, and 5 ml of Hamk’s Liquid A stock solution, 0.1 ml of 0.5% Phenol Red, and 15 pounds of autoclave for 30 minutes were injected into a Hamk’s original 5 ml of solution B, 50 mg of PHA, 0.6 ml of “20,000 mg / ml” of penicillin, 0.25 ml of streptomycin “0.5 g”, and “500 units of heparin” for “PHA injection of 10 mg for Guangzhou Pharmaceutical Industry Research Institute” Ml "3 ml. when necessary