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应用低温包埋、紫外线照射、胶体金标记、免疫电镜技术,首次在透射电镜下确定White树脂包埋淋巴细胞内β-葡葡糖醛酸酶在超微结构水平的精确定位,观察到淋巴细胞的内质网、高尔基复合体、溶酶体内散在分布着标记β-葡萄糖醛酸酶的金颗粒,表明该酶主要分布于这几种细胞器内。
Low temperature embedding, UV irradiation, colloidal gold labeling and immunoelectron microscopy were used to determine the precise localization of β-glucuronidase in White resin-embedded lymphocytes at ultrastructural level under transmission electron microscope. Lymphocytes Of the endoplasmic reticulum, Golgi complex, lysosomes scattered in the distribution of gold particles labeled β-glucuronidase, indicating that the enzyme is mainly distributed in these organelles.