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合成了槲皮素钙,观察了其生物活性。将槲皮素与醋酸钙在p H=8.5的条件下加热回流6h,生成了槲皮素钙。分析发现槲皮素钙紫外光谱带Ⅰ和带Ⅱ比槲皮素分别红移了28nm和8nm;槲皮素钙的羰基振动频率υC=O为1647.52cm-1比槲皮素向红移了15.58cm~(-1),在高场出现了新的吸收峰υM-O=640.64cm~(-1),说明有金属配位键生成;氢谱中槲皮素钙的3-OH和3’-OH上的氢消失,7-OH,5-OH,4’-OH上的氢依然存在,这说明槲皮素的3位羟基和4位羰基上的氧与钙离子配位,3’和4’位羟基与钙离子结合。用DPPH法测定了槲皮素钙与槲皮素的EC50分别是8.17和14.07mg/L,说明槲皮素钙的清除自由基的能力没有槲皮素强。
The quercetin calcium was synthesized and its biological activity was observed. Quercetin and calcium acetate were heated under reflux at pH = 8.5 for 6h to produce quercetin calcium. The results showed that quercetin calcium bands shifted to 28 nm and 8 nm, respectively. The frequency of carbonyl vibration of quercetin calcium was 1647.52 cm-1, red shifted 15.58 to quercetin cm -1, the new absorption peak υ M-O = 640.64cm -1 appeared in the high field, indicating that metal coordination bonds were formed. The 3-OH and 3 ’ Hydrogen on-OH disappeared, 7-OH, 5-OH, 4’-OH on the hydrogen still exists, which shows quercetin 3 hydroxyl and 4 carbonyl oxygen and calcium ions coordination, 3 ’and 4 ’hydroxyl and calcium ion binding. The EC50 of quercetin calcium and quercetin were determined by DPPH method respectively 8.17 and 14.07mg / L, indicating that quercetin calcium scavenging free radicals without quercetin strong.