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目的:检测高糖环境对体外培养的人牙周膜细胞(hPDLF)增殖、总蛋白合成、碱性磷酸酶活性(ALP)、Ⅰ型胶原(Col-Ⅰ)和骨钙素(OCN)分泌的影响以及胰岛素在此过程中的调节作用,为探讨糖尿病型牙周炎的发病机制及治疗方法提供理论依据。方法:采用含不同糖浓度(5.5、15、25、35、45mmol/L)的培养基培养hPDLF,各组均采用胰岛素作为治疗对照。孵育24h后,采用CCK-8法检测不同糖浓度对细胞增殖的影响;同时检测其对细胞蛋白质合成、ALP活性、Col-Ⅰ和OCN分泌的影响。采用SPSS 13.0软件包对数据进行统计学分析。结果:低浓度的葡萄糖对hPDLF增殖及其他生物学活性无显著影响,随浓度的增高,葡萄糖能够浓度依赖性抑制hPDLF的增殖,减少蛋白质合成及Col-Ⅰ分泌,抑制ALP活性及OCN分泌,这一效应可被胰岛素所抑制。结论:高糖可抑制hPDLF生物学活性,胰岛素能拮抗这种抑制作用。
AIM: To investigate the effects of high glucose on proliferation, total protein synthesis, alkaline phosphatase (ALP), collagen Ⅰ (Col-Ⅰ) and osteocalcin (OCN) secretion in cultured human periodontal ligament cells (hPDLF) Influence and regulation of insulin in this process, to provide a theoretical basis for exploring the pathogenesis and treatment of diabetic periodontitis. Methods: hPDLF was cultured in medium containing different concentrations of glucose (5.5, 15, 25, 35 and 45 mmol / L), and insulin was used as control in each group. After incubation for 24 hours, the effects of different sugar concentrations on cell proliferation were examined by CCK-8 assay. Meanwhile, the effects on cell protein synthesis, ALP activity, Col-I and OCN secretion were also examined. Data were statistically analyzed using SPSS 13.0 software package. Results: Low concentration of glucose had no significant effect on the proliferation and other biological activities of hPDLF. With increasing concentration, glucose could inhibit the proliferation of hPDLF in a concentration-dependent manner, decrease the protein synthesis and the secretion of Col-Ⅰ, inhibit the activity of ALP and the secretion of OCN One effect can be inhibited by insulin. Conclusion: High glucose can inhibit the biological activity of hPDLF, insulin can antagonize this inhibitory effect.