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目的 研究肠三叶因子 (ITF)、环氧合酶 2 (COX 2 )、诱导型一氧化氮合酶 (iNOS)在水浸束缚应激 (WRS)大鼠胃黏膜基因表达变化 ,探讨其在应激胃黏膜适应性细胞保护中的相互关系及作用。方法 采用重复WRS制作模型 ,Ⅲ型激光多谱勒血流仪 (LDF 3)动态监测胃黏膜血流量 (GMBF) ,大体及光镜下观察黏膜损伤程度 (UI)及组织学变化 ,逆转录 多聚酶链反应 (RT PCR)和Westernblot检测ITF、COX 2及iNOS表达变化。结果 单次应激造成胃黏膜广泛损伤 ,重复应激后胃黏膜产生适应性 ,胃黏膜血流量上升 ,损伤逐渐减轻 ;4次应激后 ,损伤指数降低为单次应激时的 2 1 99% ,并且胃腺区细胞增殖。单次应激后 ,ITF、COX 2及iNOS均增强 ;重复应激后 ,ITF表达继续逐渐增强 ,而COX 2及iNOS表达则逐渐减弱 ;正常时 ,ITF、COX 2及iNOS几乎无表达 ;应激后 ,ITF不但在胃腺颈部黏膜增殖带表达增强 ,而且在胃腺基底部亦有表达 ,COX 2及iNOS在溃疡及其边缘存在。结论 胃黏膜适应性细胞保护伴有细胞增殖 ,ITF表达逐渐增强 ,COX 2及iNOS表达逐渐减弱 ;表明三者在这一现象中具有重要的调节作用
Objective To investigate the gene expression changes of gastric mucosa in rats under water immersion restraint stress (WRS), and to explore the role of ITF, COX 2 and iNOS in gastric mucosa Interaction and role of stress gastric mucosal adaptive cytoprotection. Methods The model of repeated WRS was established. Gastric mucosal blood flow (GMBF) was monitored by type Ⅲ laser LDF (3), the degree of mucosal injury (UI) and histological changes were observed under light and light microscope. Reverse transcriptase polymerase The changes of ITF, COX 2 and iNOS expression were detected by RT PCR and Western blotting. Results Single stress caused extensive damage to the gastric mucosa. After repeated stress, the gastric mucosa became more adaptive and the blood flow of the gastric mucosa increased and the injury gradually decreased. After 4 times of stress, the injury index decreased to 2191 %, And gastric gland cell proliferation. ITF, COX 2 and iNOS were all increased after single stress; the expression of ITF continued to increase, while the expression of COX 2 and iNOS gradually decreased after repeated stress; almost no expression of ITF, COX 2 and iNOS at normal time; After stimulation, ITF not only enhanced the proliferation of gastric glands in the mucosa, but also expressed in the basal part of gastric glands. COX 2 and iNOS were present at the ulcer and its margins. CONCLUSIONS: Gastric mucosal adaptive cytoprotection accompanied with cell proliferation, ITF expression gradually increased, COX 2 and iNOS expression gradually decreased, indicating that the three have an important regulatory role in this phenomenon