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用 1 8 1条 1 0碱基随机引物扩增感病亲本豫麦 1 3号和含Pm6基因的抗病亲本Tim galen ,有 37条引物在Timgalen中检测到多态性片断 ,经多次重复和F2 验证 ,引物S1 38仍能在Timgalen中扩增出稳定的多态性片断 ,对该多态性片断进行回收、克隆和测序 ,根据其序列合成 1对特异引物 ,成功地将RAPD标记转化成稳定的SCAR标记 ,并用此引物对豫麦 1 3号和Timgalen的杂交F2 单株检测 ,计算出该标记与抗病基因之间的遗传距离为 2 7.1cM。并对含不同抗白粉病基因的载体品种进行了SCAR分析。
A total of 108 primers were used to amplify the resistant parents Yumai 1 3 and the resistant parent Tim galen containing Pm6 gene with 180 primers. 37 primers were used to detect the polymorphic fragments in Timgalen. After repeated And F2, the primer S1 38 could still amplify a stable polymorphic fragment in Timgalen. The polymorphic fragment was recovered, cloned and sequenced. Based on its sequence, a pair of specific primers was synthesized and the RAPD marker was successfully transformed The stable SCAR marker was used to detect the F2 hybrids of Yumai13 and Timgalen. The genetic distance between the marker and the resistance gene was calculated to be 21.7cM. SCAR analysis was carried out on the vector varieties with different powdery mildew resistance genes.